IMMUNOCYTOCHEMICAL ESTIMATION METHOD FOR PROLIFERATIVE LYMPHOCYTE STATE BY EXPRESSION BEHAVIOUR OF C-TERMINAL B23/NUCLEOPHOSMIN PROTEIN FRAGMENT IN INDIRECT IMMUNOFLUORESCENCE TEST

摘要

FIELD: medicine. ^ SUBSTANCE: immunocytochemical estimation method for proliferative lymphocyte state by an expression behaviour of an C-terminal B23/nucleophosmin protein fragment in indirect immunofluorescence test provides a visual cell estimation for count and size of luminous nucleoli marked by a specific anti-peptide antibody to the C-terminal B23/nucleophosmin protein fragment detecting a B23.1 protein oligomer form only of molecular weight 210-230 kDa. The intact lymphocyte value found in a cycling state G0 is the presence in a cell nucleus of no more than one luminous nucleoli detected by the specified C-fragment B23 antibody, while 2-3 luminous nucleoli are detected in lymphocytes in the process of proliferative lymphocyte activity caused by PHA stimulation in 24 hours from the initiation of the stimulation procedure, and 4-5 luminous nucleoli of greater size and more intense luminescence between + to +++ as compared with the reference are detected in the cells in 48-72 hours from the initiation of the stimulation procedure when most lymphocytes are found at the peak of proliferative activity in the cycling state S. ^ EFFECT: invention enables early diagnostics of malignant haematological and oncological diseases. ^ 3 dwg, 1 tbl, 2 ex