NUCLEIC ACID DETECTION BY METHOD BASED ON TARGET-SPECIFIC HYBRID LINKAGE

摘要

FIELD: medicine. ^ SUBSTANCE: what is offered is a method for target nucleic acid (NA) detection providing a) NA sample enrichment by target sequences ensured by DNA-RNA hybrid formation between the NA-target and a probe complementary at least to its site, linkage of the produced hybrids with a solid carrier and removal of the NA both/either non-hybridised and/or non-linked with a substrate; b) amplification of the target NA enriched samples and target NA detection in the produced set with the help of an oligonucleotide complementary to a first target NA site, and a probe complementary to a second target NA site where an oligonucleotide sequence and a probe sequence form with the target NA the DNA-RNA hybrid detected by any of a set of common methods. ^ EFFECT: new method is high-sensitive and high-specific and enables to distinguish between high-homologous NA sequences. ^ 22 cl, 19 tbl, 17 ex