发明名称 |
Two-photon probe for real-time monitoring of intracellular calcium ions, method for preparing the probe and method for real-time monitoring of intracellular calcium ions using the probe |
摘要 |
A two-photon probe for real-time monitoring of intracellular calcium ions is provided. The two-photon probe is very suitable for real-time imaging of intracellular calcium ions, shows 20˜50-fold TPEF enhancement in response to Ca2+, has a dissociation constant (KdTP) of 0.14±0.02 to 0.25±0.03 μM, and emits 5-fold stronger TPEF than currently available one-photon fluorescent Ca2+ probes. Unlike the previously available probes, the two-photon probe can selectively detect dynamic levels of intracellular free Ca2+ in live cells and living tissues without interference from other metal ions and from the membrane-bound probes. Moreover, the two-photon probe is capable of monitoring the calcium waves at a depth of 100-300 μm in live tissues for 1,100-4,000 s using two-photon microscopy (TPM) with no artifacts of photo-bleaching. Further provided are a method for preparing the two-photon probe and a method for real-time monitoring of intracellular calcium ions using the two-photon probe. |
申请公布号 |
US8084647(B2) |
申请公布日期 |
2011.12.27 |
申请号 |
US20080997520 |
申请日期 |
2008.01.21 |
申请人 |
CHO BONG-RAE;KIM HWAN MYUNG;KOREA UNIVERSITY INDUSTRY AND ACADEMY COOPERATIONFOUNDATION |
发明人 |
CHO BONG-RAE;KIM HWAN MYUNG |
分类号 |
C07C211/00;A61B5/00;A61B8/00;A61B10/00 |
主分类号 |
C07C211/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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