| 摘要 |
<P>PROBLEM TO BE SOLVED: To provide a new lower eukaryotic host cell producing human-like glycoproteins characterized by having a terminal β-galactose residue and essentially lacking fucose and sialic acid residues, and to provide a method for catalyzing the transfer of a galactose residue from UDP-galactose onto an acceptor substrate in a recombinant lower eukaryotic host cell, which can be used as a therapeutic glycoprotein. <P>SOLUTION: The recombinant lower eukaryotic host cell producing the human-like glycoproteins includes: an isolated nucleic acid molecule encoding the β-galactosyltransferase activity; and at least one isolated nucleic acid molecule encoding the UDP-galactose transport activity, UDP-galactose C4 epimerase activity or galactokinase activity or galactose-1-phosphate uridyl transferase. <P>COPYRIGHT: (C)2012,JPO&INPIT |
