| 摘要 |
A lab-on-a-chip (LOC) device for amplifying and detecting target nucleic acid sequences, the LOC device having restriction enzymes for restricting double stranded oligonucleotides at known ligation sites, linker molecules for attaching to the ligated ends of the double stranded oligonucleotides, heaters for thermally cycling the oligonucleotides through a polymerase chain reaction (PCR) process, primers for annealing to the linker molecules on single strands of the oligonucleotides after denaturation, deoxyribonucleoside triphosphates (dNTPs) for extending the primers along the single strand oligonucleotides, and, electrodes for receiving an electrical pulse to excite the ECL luminophores.
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