| 摘要 |
Disclosed is a technique which is for duplicating across a wide region any large region on a chromosome of a bacterium belonging to the Aspergillus strain, and which enables the stable and systematic acquisition of a previously un-acquirable bacterium belonging to the Aspergillus strain having novel traits. Disclosed is a transformant wherein a transformation marker gene sequence is arranged on the outside of terminals (5', 3') of a region so as to sandwich an arbitrary region of a chromosome, and chromosome duplication has taken place by means of homologous recombination after double strand breakage generated in a homologous region inside the gene. Also disclosed is a method for duplicating an arbitrary region of a chromosome of a bacterium belonging to the Aspergillus strain, by cultivating said transformant. |
