发明名称 |
Method for Producing Proteins |
摘要 |
This invention provides a method for obtaining a recombinant antibody with a desired function, comprising: (a) providing a population of antibody-forming cells suspected of containing at least one cell capable of producing an antibody exhibiting the desired function; (b) generating one or more transcriptionally active recombinant linear polynucleotides from the antibody forming cells obtained in step (a) wherein each transcriptionally active recombinant linear polynucleotide comprises a polynucleotide sequence encoding a variable domain of an antibody produced by an antibody-forming cell obtained in step (a) and one or more transcription regulatory elements; (c) expressing a recombinant antibody using one or more of the transcriptionally active recombinant linear polynucleotides generated in step (b); (d) screening the recombinant antibody produced by step (c) for the desired function; and (e) optionally repeating steps (b), (c) and (d) to identify a recombinant antibody exhibiting the desired function. |
申请公布号 |
US2011313138(A1) |
申请公布日期 |
2011.12.22 |
申请号 |
US201013203202 |
申请日期 |
2010.02.25 |
申请人 |
STEPHENS PAUL EDWARD;WRIGHT MICHAEL JOHN;UCB PHARMA, S.A. |
发明人 |
STEPHENS PAUL EDWARD;WRIGHT MICHAEL JOHN |
分类号 |
G01N33/53;C07K16/00 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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