Nucleotides and nucleosides having derivitized bases including a dye and terminating group and uses in DNA sequencing

摘要

Provided are nucleotides and nucleosides having formulas I-VII that can be used in DNA sequencing and analysis. The nucleotides or nucleosides have an unprotected 3'-OH group and are derivatized at the nucleobase to include a fluorescent dye attached via a linker to a non-cleavable terminating group. The non-cleavable-fluorescent group is designed to terminate DNA synthesis so that DNA oligomers can be sequenced efficiently in a parallel format. Alternatively the nucleotide or nucleoside have an unprotected 3'-OH group and are derivatized at the nucleobase to include a fluorescent dye attached via a linker to a photocleavable terminating group. The photocleavable-fluorescent group is designed to terminate DNA synthesis as well as to be cleaved so that DNA oligomers can be sequenced efficiently in a parallel format.