| 摘要 |
Discloses a method of detecting the presence of at least one pathogen comprising contacting a nucleic acid obtained from a sample with a set comprising at least four probes wherein each of said probes comprises a sequence complementary to a sequence from a pathogen flanked by four pairs of complementary bases, wherein said bases form a stem structure in the absence of hybridization to a nucleic acid from pathogen, wherein said probe is labelled with a first interacting label and a second interacting label such that hybridizing of said probe to a nucleic acid from said pathogen causes a change in the signal detected. Further discloses a set of probes comprising at least four probes wherein each of said probes comprises a sequence complementary to a sequence from a pathogen flanked by four pairs of complementary bases, wherein said bases form a stein structure in the absence of hybridization to a nucleic acid from a pathogen, wherein said probe is labelled with a first interacting label and a second interacting label such that hybridizing of said probe to a nucleic acid from said pathogen causes a change in the signal detected.
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