| 摘要 |
<p>The present invention relates to an isolated polypeptide having a-glucuronidase activity and that can degrade glucuronoxylan molecules by hydrolysis of a glycosidic linkage between a MeGIcA residue (glucuronic side chain) and a non- terminal xylopyranosyl residue. The isolated polypeptide is capable of cleaving glycosidic linkages within glucuronoxylans from plant biomass, thereby removing internal glucuronic side chains. The invention also relates to amino acid sequences of the isolated polypeptide, and homologous thereto, as well as isolated polynucleotides having nucleic acid sequences encoding the polypeptides. The invention further relates to methods of isolating the polypeptides from microbial cultures, such as that of Pichia stipitis, using chromatographic techniques, and to methods of producing substantially enriched preparations of the polypeptides.</p> |
