| 摘要 |
<p>Provided is an assay method whereby assay time can be largely shortened in the case of detecting a physiologically active substance of biological origin such as an endotoxin or ²-D-glucan or measuring the concentration of the same with the use of a reaction between said physiologically active substance and LAL. Also provided is an assay device using the same. The aggregation start time of a liquid mixture of an assay sample, which is to be subjected to the detection of a physiologically active substance or the measurement of the concentration of the same, with LAL is determined. From this aggregation start time, the physiologically active substance is detected or the concentration thereof is measured. The liquid mixture of the assay sample with LAL is stirred with, for example, a magnetic stirrer toformgelparticles.Next,thescatteredlightintensity of laser light scattered by these gel particles is measured. Then, the frequency distribution of the scattered light intensity fluctuation is obtained. Based on the temporal change in the frequency distribution shape, the aggregation start time of the liquid mixture of the assay sample with LAL is determined</p> |
