发明名称 |
Auto-inducible sodium phosphate symporter promoter from Pichia pastoris and method for producing recombinant protein using it |
摘要 |
Disclosed herein are a Pichia pastoris-derived auto-inducible NPS promoter; a recombinant expression vector carrying the promoter and a nucleotide sequence, the sequence being operably linked to the promoter and coding for a recombinant protein; a host cell transformed or transfected with the recombinant expression vector; and a method for producing a recombinant protein, comprising culturing the host cell to express the recombinant protein and isolating the protein. The NPS promoter allows a recombinant protein of interest to be produced on a large scale without the need of any inducer. |
申请公布号 |
US8080389(B2) |
申请公布日期 |
2011.12.20 |
申请号 |
US20060294511 |
申请日期 |
2006.08.07 |
申请人 |
LEE HONG WEON;AHN JUNG OH;JUNG JOON KI;CHOI EUI SUNG;KIM CHUN SUK;LEE EUN GYO;HONG JI YEON;LEE HYEOK WON;PARK MYOUNG SOO;KOREA RESEARCH INSITUTE OF BIOSCIENCE AND BIOTECHNOLOGY |
发明人 |
LEE HONG WEON;AHN JUNG OH;JUNG JOON KI;CHOI EUI SUNG;KIM CHUN SUK;LEE EUN GYO;HONG JI YEON;LEE HYEOK WON;PARK MYOUNG SOO |
分类号 |
C07H21/04;C12N1/19;C12N15/63 |
主分类号 |
C07H21/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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