SPECIFIC PRIMER FOR DIAGNOSING AGARICUS BISPORUS VIRUS ABSV, AND METHOD FOR DIAGNOSING USING THE SAME

摘要

PURPOSE: A specific primer for diagnosing Agaricus bisporus virus AbSV is provided to ensure simple and accurate detection of virus infection. CONSTITUTION: A specific primer for diagnosing Agaricus bisporus virus AbSV contains a primer pair of sequence number 1(forward primer) and sequence number 2(reverse primer). A method for diagnosing Agaricus bisporus virus AbSV infection comprises: a step of isolating ssRNA from Agaricus bisporus virus AbSV; a step of performing RT-PCR using the specific primer; and a step of analyzing the PCR product. The size of the PCR product is 700bp.