摘要 |
The present invention relates to a method for cloning double-stranded DNA (ds DNA) molecules. In particular, the present invention relates to a method for cloning ds DNA molecules using terminal transferase to tail at least one 3′ termini of the ds DNA molecules with nucleotides and ligating the tailed ds DNA molecules with a vector. Also provided are kits and compositions that can be used for cloning ds DNA molecules. |