发明名称 |
GENERATION OF RANDOM DOUBLE-STRAND BREAKS IN DNA USING ENZYMES |
摘要 |
<p>An enzyme preparation is described that includes a non-specific nuclease and a T7 Endo I mutant in a unit ratio of less than 1:200. This enzyme preparation may be used to generate double-stranded DNA fragments of a size suitable for DNA sequencing. The ends of the fragments can be readily modified as necessary to ligate adaptors or individual nucleotides to one strand of the double-stranded DNA fragments.</p> |
申请公布号 |
EP2393940(A1) |
申请公布日期 |
2011.12.14 |
申请号 |
EP20100704044 |
申请日期 |
2010.02.03 |
申请人 |
NEW ENGLAND BIOLABS, INC. |
发明人 |
HSIEH, PEI-CHUNG;GUAN, CHUDI |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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