APPARATUS AND METHOD FOR DIFFERENTIATING MULTIPLE FLUORESCENCE SIGNALS BY EXCITATION WAVELENGTH

摘要

An apparatus for differentiating multiple fluorescence signals by excitation wavelength comprises a plurality of reaction regions (10), a plurality of excitation light sources (16), a plurality of detectors (18), and a control unit. Each excitation light source is capable of providing a plurality of separate excitation beams, each excitation beam including a wavelength range that is not provided by the other excitation beams of the plurality of separate excitation beams, and each excitation light source is configured to direct each excitation beam along a beam path toward a single reaction region to which none of the other excitation light sources directs its excitation beams. Each detector detects emission beams emitted from a single reaction region. The control unit is configured to individually activate each of the plurality of excitation beams independently from activating the other ones of the plurality of excitation beams.