REGION OF INTEREST EXTRACTION AND NORMALIZATION METHODS

摘要

Aspects of the present invention are drawn to methods and compositions for the genetic analysis of regions of interest from one or more starting polynucleotide sample, e.g., a multiplexed polynucleotide sample. In certain aspects, a polynucleotide sample comprising one or more region of interest (ROI) is subjected to independent amplification reactions for specific sub-regions within the ROI(s). The amount/concentration of the product from each sub-region amplification reaction is determined followed by producing a normalized sample based on the determined amount/concentration that is suitable for further analyses (e.g., sequencing).