Methods for a microemulsion-based tissue treatment

摘要

The invention described herein is directed to the general field of anatomical pathology, and particularly to methods of preparing a paraffin-embedded biological sample for staining comprising deparaffinizing the paraffin-embedded biological sample by dissolving the paraffin with a nonpolar organic solvent and exchanging the nonpolar organic solvent in the deparaffinized tissue with a microemulsion comprising surfactant, nonpolar organic solvent, water, and optionally a polar organic cosurfactant, wherein the surfactant is soluble in both the water and the nonpolar organic solvent.