METHOD FOR TRANSFORMING SCHIZOSACCHAROMYCES POMBE, TRANSFORMANT OF SCHIZOSACCHAROMYCES POMBE, AND METHOD FOR PRODUCING HETEROLOGOUS PROTEIN

摘要

To provide a method for transforming S.pombe for creating a transformant with a high stability of maintenance after passage and enables the steady production of a heterologous protein of interest, a transformant produced by the method and a method for producing a heterologous protein using the resultant transformant. A method for transforming Schizosaccharomyces pombe by using a vector carrying an expression cassette (containing a promoter capable of functioning in Schizosaccharomyces pombe, a heterologous protein structural gene and a terminator), and having recombination region(s) at which homologous recombination with each chromosome of Schizosaccharomyces pombe is to be achieved, which comprises integrating the expression cassette into the Tf2 transposon gene position(s) in the chromosome(s) of Schizosaccharomyces pombe by homologous recombination, a transformant created by the method and a method for producing a heterologous protein using the resultant transformant.