METHOD FOR ANALYZING AMPLIFIED NUCLEIC ACIDS

摘要

The invention relates to a method for analysing nucleic acids in a microfluidic device. Said method consists of the following steps: a) nucleic acids are amplified in a first chamber in the microfluidic device; b) the amplified nucleic acids are brought into contact with an additive comprising: i) monovalent cations and ii) an Mg2+ ion-binding agent, the additive being provided in a second chamber in the microfluidic device; and c) the amplified nucleic acids are hybridised on at least one probe oligonucleotide.