摘要 |
<p>PURPOSE: A method for detecting mycobacterium tuberculosis and nontuberculous mycobacterium is provided to effectively detect and analyze the bacteria. CONSTITUTION: A probe for detecting 16S rRNA of nontuberculous mycobacterium contains a base sequence of sequence number 9. The 5' end of a probe is labeled with a fluorescent marker of FAM, VIC, TET, JOE, HEX, CY3, CY5, ROX, RED610, TEXAS RED, RED670 or NED. The 3' terminal is labeled with quencher of 6-TAMRA, BHQ-1,2,3 or MGBNFQ(molecular grove binding non-fluorescence quencher).</p> |