发明名称 Nucleic acid amplification using non-random primers
摘要 The present invention provides methods for selectively amplifying a target population of nucleic acid molecules (e.g., all mRNA molecules expressed in a cell type except for the most highly expressed mRNA species). The present invention also provides populations of oligonucleotides including the nucleic acid sequences set forth in SEQ ID NOS:1-933. These oligonucleotides can be used, for example, to prime the synthesis of cDNA molecules complementary to mRNA molecules isolated from mammalian blood without priming the synthesis of cDNA molecules complementary to globin mRNA, or ribosomal RNA molecules.
申请公布号 US2011294701(A1) 申请公布日期 2011.12.01
申请号 US201113084342 申请日期 2011.04.11
申请人 CASTLE JOHN;RAYMOND CHRISTOPHER K.;ARMOUR CHRISTOPHER;LIFE TECHNOLOGIES CORPORATION 发明人 CASTLE JOHN;RAYMOND CHRISTOPHER K.;ARMOUR CHRISTOPHER
分类号 C40B40/06;C40B50/06 主分类号 C40B40/06
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