| 摘要 |
<p>In order to remove autofluorescence emitted by a measurement object, fluorescence of the measurement object within a first wavelength band is first received. The first wavelength band is set so that the intensity of fluorescence emitted by the measurement object irradiated with intensity-modulated laser light is higher than that of autofluorescence emitted by the measurement object irradiated with the laser light. Then, the autofluorescence within a second wavelength band different from the first wavelength band is received. A generated fluorescent signal of the first fluorescence and a generated fluorescent signal of the autofluorescence are mixed with a modulation signal for modulating the laser light to produce first fluorescence data and autofluorescence data, respectively. The autofluorescence data is multiplied by a predetermined constant, and the thus obtained result is subtracted from the first fluorescence data to produce third fluorescence data. The third fluorescence data is used to calculate a fluorescence intensity.</p> |
