Virally-inactivated growth factors-containing platelet lysate depleted of PDGF and VEGF and preparation method thereof

摘要

There is an increasing interest in human platelet extracts rich in growth factors (PGF) both for wound healing and for stem cell expansion. The present invention concerns a virally-inactivated growth factors-containing platelet lysate depleted of PDGF and VEGF, which is preferably enriched in TGF, IGF and EGF-rich. The present invention further concerns a method for obtaining such a platelet lysate, comprising the steps of contacting a starting platelet concentrate with a solvent and/or a detergent, incubating said starting platelet concentrate with the solvent and/or detergent for a period of at least 5 minutes to 6 hours, at a pH maintained in a range from about 6.0 to about 9.0, and at a temperature within the range of from 2°C to 50°C, optionally removing the solvent and/or the detergent by oil extraction and obtaining an aqueous protein phase; and incubating the solvent and/or detergent-tretaed platelet concentrate or the aqueous protein phase with charcoal.