| 摘要 |
Arrays are disclosed comprising a substrate and a plurality of oligonucleotide primer spots immobilised thereon, wherein within each spot a population of primer pairs for specifically amplifying a target nucleic acid (NA) by PCR is immobilised upon the substrate such that the 3 ends of said primers can be extended, at least two of said oligonucleotide primer spots being adapted to amplify different target NAs. Methods of amplifying target NAs using the arrays are claimed (effectively utilising bridge amplification techniques). Methods of preparing oligonucleotide primer spots on an array are also claimed, wherein the methods may comprise steps of immobilising a population of a combined primer construct comprising first and second primers linked via a cleavable linkage, or wherein the methods comprise immobilising one of the primers and then covalently linking or recombining a proportion of the immobilised primers with the second primer of the primer pair.
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