SYSTHETIC POLYPEPTIDE LIBRARIES AND METHODS FOR GENERATING NATURALLY DIVERSIFIED POLYPEPTIDE VARIANTS

摘要

The invention provides compositions and methods for generating libraries of DNA sequences encoding homolo-gous polypeptides, and uses of the libraries to identify naturally diversified polypeptide variants. The invention also provides com-positions and methods for generating collections of synthetic antibody fragments in which one or several complementary deter-mining regions (CDR) are replaced by a collection of the corresponding CDR captured from a natural source. The invention fur-ther provides compositions and methods for diversifying a portion of a polypeptide by inserting a diversified sequence of synthetic or natural origin without the need for modification of the original polypeptide coding sequence.