PREPARATION OF INACTIVATED ARTIFICIAL ANTIGEN PRESENTING CELLS AND THEIR USE IN CELL THERAPIES

摘要

Disclosed is a method for creating activated T lymphocytes ex vivo for administration to a patient, comprising the steps of: inactivating Drosophila artificial antigen presenting cells (Drosophila aAPCs) with a nucleic acid crosslinking agent, wherein the nucleic acid crosslinking agent comprises a psoralen selected from the group consisting of 8-methoxypsoralen (8-MOPS), 4'- (aminomethyl)-4,5', 8-psoralen (AMT), or amotosalen (S59), and wherein said inactivating step comprises exposing the Drosophila aAPCs treated with the psoralen to a photo activating dose of UVA irradiation, contacting T lymphocytes isolated from the patient with said inactivated Drosophila aAPCs thereby creating activated T lymphocytes; and collecting the activated T lymphocytes for administration back to the patient. Also disclosed is a psoralen-inactivated Drosophila artificial antigen presenting cell (Drosophila aAPC), wherein the Drosophila aAPC expresses HLA-A2.1, B7-1 (CD80), LFA- 3(CD58), CD70 and ICAM-l (CD54) cell surface proteins and presents the following peptides: YMNGTMSQV (SEQ ID NO:5), ITDQVPFSV (SEQ ID NO:7), AAGIGILTV (SEQ ID NO:9), ELAGIGILTV (SEQ ID NO:10), SLLMWITQV (SEQ ID NO:27), FL WGPRAL V (SEQ ID NO:30), TLAKFSPYL (PRAME; SEQ ID NO:54) and VLDGLDVLL (SEQ ID NO:71).