METHOD FOR IN VITRO INDUCING MEGAKARYOCYTE PROGENITOR CELLS AND MEGAKARYOCYTES

摘要

A method for in vitro inducing megakaryocyte progenitor cells and megakaryocytes and special culture medium thereof are provided. The special culture medium is the StemSpan® serum-free medium comprising 50 ng/ml of SCF, 50 ng/ml of TPO, 20 ng/ml of IL-3, and 50 ng/ml of IL-6. The method comprises following steps: 1) separating single nuclear cells from umbilical cord bloods by the normal Ficoll density gradient centrifugation method, wherein red cells in umbilical cord bloods are settled by hydroxyethyl starch having the concentration of 6%; 2) culturing the single nuclear cells in the special culture medium for 4-14 days in vitro under the condition of 37?, 5% CO2 to obtain the mixture of megakaryocyte progenitor cells and megakaryocytes. The method is simple in operation, low in cost, and high in differentiation efficiency.