COVALENT MODIFICATION OF PROTEINS FOR THE INSTANTANEOUS VISUALISATION THEREOF AND SUBSEQUENT CHARACTERISATION THROUGH MASS SPECTROMETRY

摘要

The invention relates to the covalent modification of proteins for the instantaneous visualisation and subsequent characterisation thereof. The modification is produced by nucleophilic addition of a dye with a functional group, for example a vinyl group that reacts with the amino groups of the protein, preferably the lysine amino acids of the peptide sequence, the dye optionally having at least another functional group, for example a sulphate group that acts as an ionisable functional group to increase the water-solubility of the stain, and subsequently to increase the solubility of the peptides, influencing the mass spectometry ionisation thereof. The dye is preferably Uniblue A.