KETOREDUCTASE MUTANT

摘要

Disclosed are: a ketoreductase mutant which can be used for the efficient production of a daunorubicin derivative; DNA which encodes the mutant; a transformant which has the DNA introduced therein and therefore can produce a daunorubicin derivative; and a process for producing a daunorubicin derivative using the transformant. The ketoreductase mutant comprises an amino acid sequence produced by substituting at least one amino acid residue selected from the group consisting of amino acid residues located at positions corresponding to 42nd, 149th, 153rd, 270th and 306th amino acid residues in the amino acid sequence for a ketoreductase (EvaE) from a chloroeremomycin-producing bacterium (Amycolatopsis orientalis) by another amino acid residue.