METHOD AND KIT FOR IDENTIFYING COMPOUNDS CAPABLE OF INHIBITING HUMAN PAPILLOMA VIRUS REPLICATION

摘要

The invention provides a method and a kit for identifying compounds capable of inhibiting Human Papilloma Virus (HPV) replication. HPV genomic or subgenomic DNA is inserted into a cell line, wherein HPV DNA replication is supported, and further the influence of a compound on the replication of HPV DNA is determined. The U2OS cell line was identified as a feasible host cell line to support HPV DNA replication, and U2OS cells were identified as a suitable host for the propagation of genomes of mucosal and cutaneous tissue specific HPVs and for the HPV genome-related constructs. The method enables screening for factors inhibiting the replication of HPV DNA at different replication phases of HPV life cycle. The method can be used in pharmacological research and screening for new potential drug candidates for prevention or therapy of infections caused by various subtypes of HPV.