METHOD FOR MEASURING KINASE ACTIVITY OF CYCLIN/CDK COMPLEX

摘要

PROBLEM TO BE SOLVED: To provide methods for measuring the kinase activity of a cyclin/CDK complex to a RB protein or its partial peptide in a sample.

SOLUTION: The invention provides the methods for measuring the kinase activity of the cyclin/CDK complex to the RB protein by assaying the phosphorylation of the RB protein phosphorylated by the cyclin/CDK complex by an immunoassay method using antibody which can identify a phosphorylation condition, and methods for measuring the dephosphorylation activity to the RB protein phosphorylated by the cyclin/CDK complex. Compounds that regulate the activities of these enzymes can be screened by applying these measuring methods.

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