Process for the determination of the von Willebrand factor-cleaving activity of the ADAMTS-13 protease
摘要
Determining von Willebrand factor activity comprises mixing a sample with a human GPIbalpha protein (I) having amino acid substitutions at positions 233 and 239 and not adding ristocetin or botrocetin. Independent claims are also included for: (1) test kit for an assay as above, comprising (I) on a nonparticulate solid phase; (2) producing (I) by expressing (I) recombinantly in a prokaryotic or eukaryotic cell culture and isolating (I) from a cell lysate or culture supernatant by affinity chromatography.
申请公布号
EP2388596(A1)
申请公布日期
2011.11.23
申请号
EP20110002342
申请日期
2008.07.03
申请人
SIEMENS HEALTHCARE DIAGNOSTICS PRODUCTS GMBH
发明人
ALTHAUS, HARALD;OBSER, TOBIAS;PATZKE, JUERGEN, DR.;SCHNEPPENHEIM, REINHARD PROF.