发明名称 |
Method for activating prethrombin-1 |
摘要 |
Methods for converting prethrombin-1 to thrombin are disclosed. An aqueous solution of prethrombin-1 is applied to oscutarin-C immobilized on a solid support so as to provide from 500 mg to 4000 mg of prethrombin-1 per mL of the solid support and a contact time between the prethrombin-1 and the oscutarin-C of from 1.8 to 3.5 minutes. The resulting active thrombin may be captured on an ion exchange chromatography medium or an affinity chromatography medium.
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申请公布号 |
US8062876(B2) |
申请公布日期 |
2011.11.22 |
申请号 |
US20060097027 |
申请日期 |
2006.12.21 |
申请人 |
MALLETT ROBERT W.;STENLAND CHRISTOPHER J.;BOONE JONATHON C.;FORSTROM JOHN W.;DE JONGH KAREN S.;ZYMOGENETICS, INC. |
发明人 |
MALLETT ROBERT W.;STENLAND CHRISTOPHER J.;BOONE JONATHON C.;FORSTROM JOHN W.;DE JONGH KAREN S. |
分类号 |
C12N9/74 |
主分类号 |
C12N9/74 |
代理机构 |
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代理人 |
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地址 |
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