METHOD OF OBTAINING DRY FORM OF BIOPREPARATION FOR CLEANING TERRITORIES FROM OIL CONTAMINANTS AND OIL PRODUCTS

摘要

FIELD: chemistry. ^ SUBSTANCE: disclosed is a method of obtaining a dry form of a biopreparation based on Pseudomonas or Rhodococcus oil-decomposing microorganism for cleaning territories from oil and oil products. Pseudomonas or Rhodococcus bacteria are cultured in a liquid culture medium. The bacteria suspension is them mixed with a protective medium. The protective medium is prepared based on 0.05 M sodium-potassium phosphate buffer with pH 6.8, with the following ratio of components: 4% polyglucin, 10% saccharose, 4% thiourea, 2% ascorbic acid, followed by titration with 45% sodium hydroxide solution to pH 6.8-7.2. The obtained mixture is added to a sorbent - expanded pearlite sand. The preparation then undergoes contact drying at t=37C until constant mass. ^ EFFECT: method increases survival rate of bacterial cells of oil-decomposing microorganisms Pseudomonas and Rhodococcus. ^ 1 dwg, 2 tbl, 3 ex