PROCESS FOR REGULATION OF MOUSE EMBRYONIC STEM CELLS PROLIFERATION THROUGH HIGH GLUCOSE-INDUCED FIBRONECTIN SYNTHESIS

摘要

PURPOSE: A method for enhancing fibronectin using glucose of high concentration is provided to stimulate G1 progress and to proliferate embryonic stem cells. CONSTITUTION: A method for enhancing fibronectin synthesis is performed through angiotensin II and deformability growth factor-beta1(TGF-beta1) in case of treating mouse embryonic stem cells with 25mM or more of glucose. The glucose of high concentration activates angiotensin II type 1 receptor. The mouse embryonic stem cells are ES-E14TG2a or ES-R1. A medium for proliferating mouse embryonic stem cells contains 25mM or more of glucose.