HIGH THROUGHPUT PAIRED-END SEQUENCING OF LARGE-INSERT CLONE LIBRARIES
摘要
The present invention is related to genomic nucleotide sequencing. In particular, the invention describes a paired end sequencing method that improves the yield of long-distance genomic read pairs by constructing long-insert clone libraries (i.e., for example, a fosIll library or a fosCN library) and converting the long-insert clone library using inverse polymerase chain reaction amplification or shearing and recircularization of shortened fragments into a library of co-ligated clone-insert ends. The resultant jumping libraries are compatible with massively parallel sequencing techniques. The compositions and methods disclosed herein contemplate sequencing complex genomes as well as detecting chromosomal structural rearrangements.
申请公布号
WO2011143231(A2)
申请公布日期
2011.11.17
申请号
WO2011US35940
申请日期
2011.05.10
申请人
THE BROAD INSTITUTE;GNIRKE, ANDREAS;NICOL, ROBERT;WILLIAMS, LOUISE;COSTELLO, MAURA, T.;STEELMAN, SCOTT
发明人
GNIRKE, ANDREAS;NICOL, ROBERT;WILLIAMS, LOUISE;COSTELLO, MAURA, T.;STEELMAN, SCOTT