ISOTHERMAL AMPLIFICATION OF NUCLEIC ACID USING PRIMERS COMPRISING A RANDOMIZED SEQUENCE AND SPECIFIC PRIMERS AND USES THEREOF

摘要

Methods and kits for amplifying a nucleic acid under isothermal conditions to form an amplified nucleic acid sequence are provided. The methods and kits comprises providing a nucleic acid template, a DNA polymerase, deoxyribonucleoside triphosphates, a primer comprising a randomized sequence, and a specific primer, and amplifying the nucleic acid template.