Method for extracellular production of recombinant biomolecules in a microorganism

摘要

The present invention relates to a method for producing into the extracellular medium a biomolecule of interest, the method comprising the steps of:
(a) culturing a recombinant microorganism co-expressing:
- the mutant pIV S324G porine into the outer membrane of the microorganism and
- a recombinant biomolecule of interest into the periplasm of the microorganism,
thereby secreting the biomolecule of interest into the extracellular medium; and
(b) recovering the biomolecule of interest from the extracellular medium.