REAGENZIEN, KITS UND VERFAHREN ZUM NACHWEIS BIOLOGISCHER MOLEKÜLE MITTELS ENERGIEÜBERTRAGUNG VON EINEM AKTIVIERTEN CHEMILUMINESZENZSUBSTRAT AUF EINEN ENERGIEAKZEPTORFARBSTOFF

摘要

Reagents, kits and methods for detecting biological molecules by energy transfer from an activated chemiluminescent substrate to an energy acceptor dye such as a J-aggregated dye are described. A kit for detecting analyte in a sample comprises: an article of manufacture comprising a support having a surface, an energy acceptor dye on the surface of the support, and one or more biomolecular probes on the surface of the support, wherein the one or more biomolecular probes is capable of binding to the analyte or, when the analyte is present, which binds to the analyte; a chemiluminescent substrate; and optionally, an enzyme-labeled biomolecule or enzyme-labeled analyte.