GLUTAMINE-AUXOTROPHIC HUMAN CELL CAPABLE OF PRODUCING PROTEIN AND CAPABLE OF GROWING IN GLUTAMINE-FREE MEDIUM

摘要

PROBLEM TO BE SOLVED: To provide a novel glutamine-auxotrophic human cell capable of producing a protein and capable of growing in a glutamine-free medium.

SOLUTION: The glutamine-auxotrophic human cell transfected with an exogenous DNA sequence encoding a protein or an exogenous DNA sequence capable of altering the expression of an endogenous gene encoding a protein and an exogenous DNA sequence encoding a glutamine synthetase, wherein these exogenous DNA sequences are located on one or more DNA construct, and the transfected cell is capable of producing the protein and capable of growing in the glutamine-free medium.

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