发明名称 |
SILICA MAGNETIC PARTICLES WITH A HIGH NUCLEIC ACID BINDING CAPABILITY |
摘要 |
<p>Magnetic particles for nucleic acid isolation are coated with silica and separated from impurities and nanoparticulates using a multi-step fractionation process. In each cycle of the fractionation process, the particles are stirred, sedimented, and resuspended, resulting in a decline in pH of the suspended particles. Repeating the fractionation process until the resuspended particles have dropped to a target pH in the range of about 9 to 10.5, and their zeta potential is more negative than about−40 mV, results in a purified population of particles with a high and reproducible binding capacity for nucleic acids. The silica-treated magnetic beads produced by the method offer improved sensitivity and consistency for recovery of nucleic acids in a sample.</p> |
申请公布号 |
EP2125659(A4) |
申请公布日期 |
2011.11.09 |
申请号 |
EP20080714166 |
申请日期 |
2008.02.01 |
申请人 |
SIEMENS HEALTHCARE DIAGNOSTICS INC. |
发明人 |
SHERMAN, DAVID;HILDENBRAND, KARLHEINZ |
分类号 |
C04B35/00;C09C1/24;G01N33/53;G01N33/543;G01N33/552;H01F1/00;H01F1/36 |
主分类号 |
C04B35/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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