Method of producing biologically active vitamin K dependent proteins by recombinant methods
摘要
The invention relates to commercially viable methods for producing biologically active vitamin K dependent proteins, particularly Factor IX. Factor IX is produced at a level of at least about 15mg/L and is at least 25% biologically active. The method relies upon co-expression of one or more of paired basic amino acid converting encyme (PACE), vitamin K dependent epoxide reductase (VKOR) and vitamin K dependent ³-glutamyl carboxylase (VKGC) at a preferred ratio so that the vitamin K dependent protein is efficiently produced and processed by a recombinant cell.
申请公布号
EP2385125(A2)
申请公布日期
2011.11.09
申请号
EP20110157194
申请日期
2006.12.21
申请人
INSPIRATION BIOPHARMACEUTICALS, INC.;UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL
发明人
DROHAN, WILLIAM N.;GRIFFITH, MICHAEL J.;TAYLOR, JOHN R.;STAFFORD, DARREL W.