SWITCH MODE NUCLEIC ACID APTAMER PROBE AND USES THEREOF IN DETECTION OF TUMOR LIVING CELL AND LIVING BODY

摘要

The invention belongs to the nucleic acid detection technical field, in particular discloses a switch mode nucleic acid aptamer probe and uses thereof. The nucleic acid aptamer probe comprises a probe main body as well as a fluorescence generating unit and a fluorescence extinguishing unit which are respectively connected with two ends of the probe main body, the probe main body comprises an aptamer fragment with a function of specific recognition for target tumor cell and a nucleic acid fragment forming complementary sequence with the aptamer fragment, and the aptamer fragment and the nucleic acid fragment are linked by a connection fragment with a length of 7-15 nm so as to form a hairpin structure; and the ability of competitive hybridization of the nucleic acid fragment with the aptamer fragment is weaker than that of the target tumor cell. The use of the probe of the invention can be at least one of specificity detection of tumor living cell in buffer solution, effective detection of tumor living cell in serum, and real-time fluorescence imaging and intravital detection of tumor in living body.