This invention relates to a cell culture process for the production of polypeptides in mammalian CHO cells characterized by one or more temperature and pH shifts which are adjusted in respect to their timing and step size to reduce cell death, increase product yield and improve product quality.
申请公布号
WO2011134919(A2)
申请公布日期
2011.11.03
申请号
WO2011EP56507
申请日期
2011.04.25
申请人
NOVARTIS AG;JOOSTEN, CHRISTOPH E.;LEIST, CHRISTIAN;SCHMIDT, JOERG
发明人
JOOSTEN, CHRISTOPH E.;LEIST, CHRISTIAN;SCHMIDT, JOERG