CORE-SHELL STRUCTURED DELIVERY SYSTEM FOR GROWTH FACTORS, A PREPARATION METHOD THEREOF, AND USE THEREOF FOR THE DIFFERENTIATION OR PROLIFERATION OF CELLS

摘要

PURPOSE: A method for preparing a delivery system for supporting physiologically active growth factors which are necessary for cell differentiation and proliferation is provided to maximize stem cell differentiation ability in vitro. CONSTITUTION: A growth factor delivery system comprises a core and shell structure containing two kinds of ingredients which are necessary for cell differentiation or proliferation. The delivery system is in the form of a microsphere of 100-400 um in dianeter. The delivery system is treated with a crosslinking agent of ethyl dimethyl amino propyl carbodiimide, genipin, or glutaraldehyde in the shell. A method for preparing the delivery system comprises: a step of preparing a polymeric microsphere containing a first ingredient needed for cell differentiation or proliferation; and a step of introducing the polymeric microsphere into other polymers containing a second ingredient needed for cell differentiation or proliferation.