| 摘要 |
Disclosed is a method to determine the effect of a treatment on viability of endothelial cells as compared to non-endothelial cells in a microaggregate. The method comprises (a) Contacting a composition comprising at least one microaggregate comprised of viable endothelial cells and viable non-endothelial cells with said treatment, (b) Adding a first indicator dye which is taken up by non-living cells and excluded by living cells; (c) Sedimenting said microaggregate onto a surface for microscopic observation; (d) Determining by microscopic observation the effect of said treatment on the viability specifically of endothelial cells in said microaggregate wherein non-viable endothelial cells are identified as hyperchromatic cells thus distinguishable from non-endothelial non-viable cells; A treatment that is determined to effect endothelial cell death is identified as successful in killing endothelial cells.
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