ANTI-MICROBIAL AGENT FROM PAENIBACILLUS SP. AND METHODS AND USES THEREOF

摘要

The present invention provides, in part, a Paenibacillus sp isolate, designated Paenibacillus polymyxa JB05-01-1, and deposited at the American Type Culture Collection (ATCC) under the accession number PTA-10436. The partial sequence of the 16S rRNA gene of P. polymyxa JB05-01-1 (i e, SEQ ID NO 1) is provided. Also provided are compositions, methods, and uses featuring the bacterium, as well as an anti-microbial agent obtained from the bacterium or cell culture supernatant thereof. The anti-microbial agent inhibits various gram- negative bacteria (e g, E. coli) as well the gram-positive bacterium Listeria innocua. The anti-microbial agent is sensitive to various proteases and lipase, sensitive to heating at 100°C for 10 minutes, insensitive to heating at 80°C for 30 minutes, and insensitive to pH ranging from 2 to 9. The size of the anti-microbial agent is less than 2500 Da.