<p>The present invention discloses a liquid chip for detecting KRAS gene mutation, comprising: (A) wild-type and mutant-type ASPE primers directed to mutant sites in KRAS gene; (B) microspheres coated with specific anti-tag sequences and having various color codes, and (C) amplification primers for amplifying the target sequences containing mutant sites in Codon 12, Codon 13 and/or Codon 61 of KRAS gene.</p>
