| 摘要 |
In order to determine the distribution of a substance in a measuring region, wherein an optical signal (1) can be used to transfer the substance (a) from one state, in which no measurement signal (3) can be obtained from the substance, into a measurement state, in which a measurement signal (3) can be obtained from the substance, and another optical signal (5) can be used to transfer the substance (b) into the one state or into a further state, in which no measurement signal can be obtained from the substance, a measuring front (4) is formed in the measuring region by structuring the optical signal (1) and the further optical signal (5) in the same manner, wherein the intensity of the optical signal (1) and of the further optical signal (5) increases over a depth of the measuring front (4), which is lower than the diffraction limit at the wavelength of the optical signal (1) and of the further optical signal (5), in such a manner that the proportion of the substance (10) in the measurement state increases from "not present" as a result of the substance being transferred (a) from the one state into the measurement state and falls to "not present" again as a result of the substance being transferred (b) into the one state or into the further state. The measuring front (4) is moved across the measuring region in the opposite direction to the increase in the intensity of the optical signal (1) and of the further optical signal (5). The measurement signal (3) is detected at least from the region of the measuring front (4) and is assigned to associated positions of the measuring front (4) in the measuring region. |
